Amanda Cristina Morais de Castro, Rachel Maciel Monteiro, Pedro Castania Amadio Domingues, Marinila Buzanelo Machado, Ana Maria Razaboni, Evandro Watanabe


Objective: To evaluate bacterial load of water using and not using sodium hypochlorite aiming at bacterial control of dental unit water. Method: Dental unit reservoirs from a dental clinic from School of Dentistry of Ribeirão Preto -USP were filled with public-supply water with and without sodium hypochlorite solution at 0.0003%, and water samples were collected and evaluated by Petrifilm™ AC (Aerobic Count) system from 2014 to 2017. Results: No bacterial contamination was observed in the water samples using sodium hypochlorite solution. However, bacterial contamination was detected in water from reservoirs, air-water syringes and high-speed outputs (without handpieces) without the disinfectant. Conclusion: The use of sodium hypochlorite solution controlled bacterial load of all the dental unit water samples as established by Brazilian legislation (<500CFU/mL).


Biofilms; Dental Equipment; Water Microbiology.

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