Sarah Raquel De Annunzio, Filipe Silveira Fusco, Carolina Santezi Santezi, Bárbara Donadon Reina, Lívia Nordi Dovigo


Objective: to evaluate the concordance of different plating methods for quantification of Candida species colonies. Method: standardized suspensions of reference strains (Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei) were submitted to serial dilution and plating according to methods of track-dilution (TDM), drop plate (DPM) and the conventional spread plate (SPM). Data were submitted to construction of Bland–Altman diagrams, Intraclass Correlation Coefficient (ICC) and ANOVA (⍺=5%). Results: adequate concordance between the methods (CCI >0.71) was observed, and the execution of DPM was the fastest (p<0.001). However, DPM and TDM appear to result in greater values compared to SPM, especially for C. tropicalis and C. krusei. Conclusion: C. albicans and C. glabrata can be plated with DPM and TDM, but the use of these methods for C. krusei and C. tropicalis may result in count variation.


Candida albicans; Candida tropicalis; Candida glabrata; Confiabilidade dos Dados; Contagem de Colônia Microbiana

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Millsop JW, Fazel N. Oral candidiasis. Clin Dermatol [Internet]. 2016 Jul [cited 2019 Dec 16]; 34(4):487-94. Available from: http://dx.doi.10.1016/j.clindermatol.2016.02.022

Kalantar E, Marashi SM, Pormazaheri H, Mahmoudi E, Hatami S, Barari MA, et al. First experience of Candida non-albicans isolates with high antibiotic resistance pattern caused oropharyngeal candidiasis among cancer patients. J Cancer Res Ther [Internet]. 2015 Apr [cited 2019 Dec 16]; 11(2):388-90. Available from: http://dx.doi.0.4103/0973-1482.157307

Cortegiani A, Misseri G, Chowdhary A. What's new on emerging resistant Candida species.Intensive Care Med [Internet]. 2019 Apr [cited 2019 Dec 16]; 45(4):512-515. Available from:

Taei M, Chadeganipour M, Mohammadi R. An alarming rise of non-albicans Candida species and uncommon yeasts in the clinical samples; a combination of various molecular techniques for identifcation of etiologic agents. BMC Res Notes [Internet]. 2019 Nov [cited 2019 Dec 16]; 12:779-89. Available from:

Brunetti G, Navazio AS, Giuliani A, Giordano A, Proli EM, Antonelli G, et al. Candida blood stream infections observed between 2011 and 2016 in a large Italian University Hospital: A time-based retrospective analysis on epidemiology, biofilm production, antifungal agents consumption and drug-susceptibility. Plos one [Internet]. 2019 Nov [cited 2019 Dec 16]; 4(11):1-10. Available from:

Antinori S, Milazzo L, Sollima S, Galli M, Corbellino M. Candidemia and invasive candidiasis in adults: A narrative review.Eur J Intern Med [Internet]. 2016 Oct [cited 2019 Dec 16]; 34:21-28. Available from:

Fernández-Ruiz M, Puig-Asensio M, Guinea J, Almirante B, Padilla B, Almela M, et al. Candida tropicalis bloodstream infection: Incidence, risk factors and outcome in a population-based surveillance. J Infect [Internet]. 2015 Sep [cited 2019 Dec 16]; 71(3):385-94. Available from:

Carmello JC, Dovigo LN, Mima EG, Jorge JH, de Souza Costa CA, Bagnato VS, et al. In vivo evaluation of photodynamic inactivation using Photodithazine® against Candida albicans. Photochem Photobiol Sci [Internet]. 2015 Jul [cited 2019 Dec 16]; 14(7):1319-28. Available from: http://dx.doi.org10.1039/c4pp00368c

Dovigo LN, Carmello JC, de Souza Costa CA, Vergani CE, Brunetti IL, Bagnato VS, et al. Curcumin-mediated photodynamic inactivation of Candida albicans in a murine model of oral candidiasis. Med Mycol [Internet]. 2013 Apr [cited 2019 Dec 16]; 51(3):243-51. Available from: http://dx.doi.10.3109/13693786.2012.714081

Taff HT, Nett JE, Andes DR. Comparative analysis of Candida biofilm quantitation assays. Med Mycol [Internet]. 2012 Apr [cited 2019 Dec 16]; 50(2):214-8. Available from: http://dx.doi.10.3109/13693786.2011.580016

Thomas P, Sekhar AC, Mujawar MM. Nonrecovery of varying proportions of viable bacteria during spread plating governed by the extent of spreader usage and proposal for an alternate spotting-spreading approach to maximize the CFU. J Appl Microbiol [Internet]. 2012 Aug [cited 2019 Aug 30]; 113(2):339-50. Available from:

Jett BD, Hatter KL, Huycke MM, Gilmore MS. Simplified agar plate method for quantifying viable bacteria. Biotechniques [Internet]. 1997 Oct [cited 2019 Aug 30]; 23(4):648-50. Available from:

Herigstad B, Hamilton M, Heersink J. How to optimize the drop plate method for enumerating bacteria. J Microbiol Methods [Internet]. 2001 Mar [cited 2019 Aug 30]; 44(2):121-29. Available from:

Sieuwerts S, De Bok FA, Mols E, De Vos WM, Vlieg JE. A simple and fast method for determining colony forming units. Lett Appl Microbiol [Internet]. 2008 Oct [cited 2019 Aug 30]; 47(4):275-8. Available from: http://dx.doi.10.1111/j.1472-765X.2008.02417.x

Hamilton CM, Anderson M, Lape J, Creech E, Woessner J. Multichannel plating unit for high-throughput plating of cell cultures. Biotechniques [Internet]. 2002 Sep [cited 2019 Aug 30]; 33(2):420-3. Available from:

McNulty JJ, Dunn JJ. High-throughput transformation and plating using petristrips. Biotechniques [Internet]. 1999 Aug [cited 2019 Aug 30]; 26(3):390-2. Available from:

Tornero P, Dangl JL. A high-throughput method for quantifying growth of phytopathogenic bacteria in Arabidopsis thaliana. Plant J [Internet]. 2002 Jan [cited 2019 Aug 30]; 28(4):475-81. Available from:

Barbosa HR, Rodrigues MF, Campos CC, Chaves ME, Nunes I, Juliano Y, et al. Counting of viable cluster-forming and non cluster-forming bacteria: A comparison between the drop and the spread methods. J Microbiol Methods [Internet]. 1995 Apr [cited 2019 Aug 30]; 22(1):39-50. Available from:

Robinson SE, Wright EJ, Williams NJ, Hart CA, French NP. Development and application of a spiral plating method for the enumeration of Escherichia coli O157 in bovine faeces. J Appl Microbiol [Internet]. 2004 Jun [cited 2019 Aug 30]; 97(3):581-9. Available from:

Dai T, Garcia B, Murray CK, Vrahas MS, Hamblin MR. UVC light prophylaxis for cutaneous wound infections in mice. Antimicrob Agents Chemother [Internet]. 2012 Jul [cited 2019 Aug 30]; 56(7):3841-8. Available from:

Stamps JA, Yang LH, Morales VM, Boundy-Mills KL. Drosophila Regulate yeast density and increase yeast community similarity in a natural substrate. PLoS One [Internet]. 2012 Jul [cited 2019 Aug 30]; 7(7):1-12. Available from:

Garcez AS, Nuñez SC, Hamblin MR, Ribeiro MS. Antimicrobial effects of photodynamic therapy on patients with necrotic pulps and periapical lesion. J Endod [Internet]. 2008 Feb [cited 2019 Aug 30]; 34(2):138-42. Available from:

Kato IT, Prates RA, Sabino CP, Fuchs BB, Tegos GP, Mylonakis E, et al. Antimicrobial photodynamic inactivation inhibits Candida albicans virulence factors and reduces in vivo pathogenicity. Antimicrob Agents Chemother [Internet]. 2013 Jan [cited 2019 Aug 30]; 57(1):445-51. Available from:

Viljoen A, Twomey PJ. True or not: uncertainty of laboratory results. J Clin Pathol [Internet]. 2007 Jun [cited 2019 Aug 30]; 60(6):587-8. Available from:

Walsh TJ, Venanzi WE, Dixon DM. Quantification of medically important Candida species and Torulopsis glabrata by a spiral inoculation system: correlation with pour plate and spread plate methods. J Clin Microbiol [Internet]. 1985 Nov [cited 2019 Aug 30]; 22(5):745-7. Available from:

Dovigo LN, Pavarina AC, Ribeiro AP, Brunetti IL, Costa CA, Jacomassi DP, et al. Investigation of the photodynamic effects of curcumin against Candida albicans. Photochem Photobiol [Internet]. 2011 Apr [cited 2019 Aug 30]; 87(4):895-903. Available from:

Chen CY, Nace GW, Irwin PL. A 6x6 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli. J Microbiol Methods [Internet]. 2003 Nov [cited 2019 Aug 30]; 55(2):475-9. Available from:

McGraw KO, Wong SP. Forming inferences about some intraclass correlations coefficients. Psychol Methods [Internet]. 1996 Jan [cited 2019 Aug 30]; 1(1):30-46. Available from:

Fermanian J. Mesure de l’accord entre deux juges. Cas qualitatif. Rev Epidemiol Sante Publique [Impresso]. 1984 [cited 2019 Aug 30]; 32(2):140-7.

Bland JM, Altman DG. Measuring agreement in method comparison studies. Stat Methods Med Res [Internet]. 1999 Apr [cited 2019 Aug 30]; 8(2):135-160. Available from:

Telles DR, Karki N, Marshall MW. Oral Fungal Infections. Dent Clin North Am [Internet]. 2017 Apr [cited 2019 Aug 30]; 61(2):319–349. Available from:

Paulone S, Malavasi G, Ardizzoni A, Orsi CF, Peppoloni S, Neglia RG, et al. Candida albicans survival, growth and biofilm formation are differently affected by mouthwashes: an in vitro study. New Microbiol [Internet]. 2017 Jan [cited 2019 Dec 16]; 40(1):45-52. Available from:



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